杰多霉素外排泵基因功能研究应用及一种biosensor筛选新调控基因方法的建立 

由于抗生素所具有的应用和商业价值,理性改造菌株、提高产量一直是抗生素的研究重点。本论文依据研究内容不同分为两部分。第一部分:易化扩散家族(MFS)转运蛋白是微生物中的一种膜蛋白,通常负责抗生素的外排。本文以杰多霉素生物合成簇内MFS家族蛋白JadL为例,采用基因敲除、回补、过表达等基因工程方法,首次展开这种独立存在于抗...
兰州大学  硕士论文  2012年 下载次数(5)| 被引次数()

损耗波生物传感器实时监控多聚酶DNA的交互反应(英文) 

Recent advances in biosensor technology have contributed significantly to our understanding of the kinetic basis of molecular recognition processes.Biosensor...
生命科学与生物技术:中国科协第三届青年学术年会论文集  1998-08-20 下载次数(17)| 被引次数(0)

Spatio-temporal analysis for phosphatidic acid at plasma membrane in plant cells using a FRET-based biosensor 

Phosphatidic acid(PA) is a structurally simplest membrane phospholipid,which plays key roles in membrane trafficking,stress responses and cytoskeletal dynamics....
2016年全国植物生物学大会摘要集  2016-10-09 下载次数(7)| 被引次数(0)

Assembly of nanoparticles into miniaturized capacitive biosensor based on interdigitated array microelectrodes for multiplexed proteins detection 

Highly sensitive,selective and rapid immunoassays capable of detecting proteins in a multiplexed fashion are essential for disease diagnostics,drug screening...
第二届全国“跨学科蛋白质研究”学术讨论会论文集  2008-07-01 下载次数(22)| 被引次数(0)

The simultaneous detection of pathogenic bacteria by a long-range surface Plasmon resonance(LRSPR) biosensor based on multichannel microfluidic system 

Rapid and sensitive detection of pathogenic bacteria is of great importance for food industry, environmental monitoring and clinical diagnosis.Herein,we used...
广东省生物物理学会2013年学术研讨会论文集  2013-12-06 下载次数(10)| 被引次数(0)

环境微生物与环境污染物的相互作用机制及环境行为 

虽然环境污染物对环境健康的影响已非常重视,但是污染物对环境的影响至今仍然是一个难以解决的问题,环境污染物既包括了传统的重金属、农药和其它的有机有害物,也包括了新型的污染物(比如碳纳米材料)。这些污染物进入环境,势必会对环境微生物产生不利的影响。污染物之间也会因不同的物理化学性质而相互作用,从而改变其环境行为...
中国地质大学  博士论文  2010年 下载次数(2243)| 被引次数(4)

Approach to Quantitative Detection of CD146 with a Label-free Protein Biosensor Based on Imaging Ellipsometry 

CD146 glycoprotein, belonging to the immunoglobulin gene superfamily, plays a unique role in heterophilic cell-cell interactions as a calcium ions independen...
《生物物理学报》  2009年 第S1期 下载次数(25)| 被引次数(0)

Detection of Peptide Damage Induced by Fenton Reaction with Electrochemical Biosensor 

Hydroxyl radical is one of the most reactive oxygen species (ROS) in biological systems, which can react with proteins and result in protein oxidation and da...
《生物物理学报》  2009年 第S1期 下载次数(28)| 被引次数(0)

A Simple and Reliable Assay for Detecting Specifi c Nucleotide Sequences in Plants Using Optical Thin-fi lm Biosensor Chips 

Here we report the adaptation and optimization of an effi cient, accurate and inexpensive assay that employs custom-designed silicon-based optical thin-fi lm...
《分子植物育种》  2007年 第02期 下载次数(28)| 被引次数(1)

Biosensor Based HTS Method for Molecular Evolutionary of the Organophosphate Hydrolase 

Organophosphates(OPs) are highly toxic synthetic compounds and are widely used as pesticides,plasticizers and flame retardants.Various enzyme were reported to d...
第十一届中国酶工程学术研讨会论文摘要集  2017-10-18 下载次数(6)| 被引次数()

Construction fusion protein with potential use in biosensor by conjugation of scFV and invertase 

The construction of recombinant by conjugation of Single Chain Fragment Variable(scFV)and invertase gene,combined with separating the space steric hindrance ...
第九届中国酶工程学术研讨会论文摘要集  2013-11-14 下载次数(2)| 被引次数(0)

A microarray biosensor based on imaging ellipsometry and its applications 

The concept of biosensor based on imaging ellipsometry and its primal working system was reported in 1995. Since then, some microarray biosensors for biomedical...
《中国医学工程》  2005年 第02期 下载次数(9)| 被引次数(1)

由正交融合蛋白激活的酿酒酵母合成启动子库的构建 

合成生物学是一门结合工程学和分子生物学知识的复合新型学科。生物感应器是合成生物学的主要研究内容之一。生物感受器是利用工程化改造后的菌种检测细胞内化合物浓度,通过输出特殊信号,如表达荧光蛋白,来表征化合物浓度或者是通过感应化合物激活某些代谢通路来实现特定功能,如聚集并降解检测物。从环境污染物清除到体内病变细胞的检测,生物...
哈尔滨工业大学  硕士论文  2018年 下载次数(25)| 被引次数()

An aptamer-based biosensor for colormetric detection of salmonella typhimurium 

Objective:To develop an aptamer based biosensor (aptasensor) for specic detection of Salmonella typhimurium. Methods:(1) The aptasensor was fabricated by mod...
中国生物化学与分子生物学会第十届会员代表大会暨全国学…  2010-08-23 下载次数(9)| 被引次数(0)

Polystyrene Based SPR Biosensor Chip for Use in Immunoassay 

Biosensors are widely used in immunoassay. The biosensor chip carries a receptor which is used in immunoassay and the chip properties have an important influenc...
《Tsinghua Science and Technology》  1999年 第03期 下载次数(29)| 被引次数(3)

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